Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)
نویسندگان
چکیده
The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds Philippines and determined its association with subclinical mastitis (SCM). involved collection of milk at second week lactation (n=75) blood samples (n=5) to obtain extracted RNA using cDNA amplify Lf through polymerase chain reaction. nucleotide amino acid sequences were used as reference evaluation phylogenetic relationship. Amplified products utilized for RFLP analysis before determining SCM. Results demonstrated that goats registered a molecular weight 2135. Nucleotide sequence revealed high similarity (99%) Saanen, Anglo-Nubian Philippine native Capra hircus (U53857) submitted GenBank. Phylogenetic studies showed Anglo-Nubian, Saanen Native clade together C. (U53857). Three genotypes documented restriction enzymes AluI HaeIII. Based on Statistical analysis, (comp 5.65, p = 0.0308) has been established between genes genotype BB SCM HaeIII enzyme.
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ژورنال
عنوان ژورنال: Acta Scientiarum. Animal Sciences
سال: 2022
ISSN: ['1806-2636', '1807-8672']
DOI: https://doi.org/10.4025/actascianimsci.v44i1.56368